Table 7.
A PCR program for denaturation procedure
| Step | Temperature | Time | Ramping Speed |
|---|---|---|---|
| 1 | 95°C | 5min | NA |
| 2 | 95°C-85°C | NA | -2°C/sec |
| 3 | 85°C-25°C | NA | -0.1°C/sec |
| 4 | 4°C | ∞ | NA |
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