(A) Specificity study of CT targeting ssDNA‐AuNPs in presence of 1. Chlamydia trachomatis (Ct = 25), 2. Chlamydia trachomatis and Neisseria gonorrhoeae (Ct = 30), 3. Staphylococcus aureus, 4. Acinetobacter baumannii, 5. Escherichia coli, 6. Bacillus subtilis, 7. Streptococcus mutans and 8. negative control sample (water). (B) Specificity study of NG targeting ssDNA‐AuNPs in presence of 1. Neisseria gonorrhoeae (Ct = 27), 2. Chlamydia trachomatis and Neisseria gonorrhoeae (Ct = 32), 3. Staphylococcus aureus, 4. Acinetobacter baumannii, 5. Escherichia coli, 6. Bacillus subtilis, 7. Streptococcus mutans and 8. negative control sample (water). Data are presented as mean ± SD. Error bar indicates the measurements from three (n = 3) such independent experiments.