Table 2.

Protocol for polymerase chain reaction (PCR) and the thermocycling conditions used in the process.

PCR step	Temperature	Time
Initial Denaturation	95 °C	30 s
45 Cycles	95 °C	30 s
	68 °C	150 s (2:30 mins)
Final Extension	68 °C	5 min
Hold	4 °C	∞