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Protocol for quantitative polymerase chain reaction (qPCR) and the thermocycling conditions used in the process.
| PCR step | Temperature | Time | Cycles |
|---|---|---|---|
| Initial Denaturation | 95 °C | 60 s | 1 |
| Denaturation | 95 °C | 15 s | 45 |
| Extension | 60 °C | 30 s | |
| Melt Curve | 60–95 °C | various | 1 |
