FIG. 1.

Locations of restriction sites in B. megaterium gpr and possible catalytic residues in B. megaterium GPR (A) and amino acid sequences of B. megaterium GPR variants (B). (A) The locations of restriction sites and codons for possible catalytic residues in B. megaterium gpr were taken from reference 30. The locations of codons for possible catalytic residues are given above the gene (open box), and restriction sites (C1, ClaI; E1, EagI; H2, HincII; H3, HindIII; NcI, NcoI; NrI, NruI) are indicated below the gene. N and C denote the N- and C-terminal coding regions, respectively. Note that the HincII and HindIII sites are outside the gpr gene’s coding sequence. The arrow labeled ΔP₃₉ denotes the region in which P₄₁ autoprocesses to P₃₉. (B) Amino acid sequences (in one-letter code) of various forms of GPR. The sequences of P₄₆ and P₄₁ (note that this is P₄₁ overexpressed in E. coli) are from references 6 and 24. The sequence of ΔP₃₉ is from this work, and those of the variants lacking 3 to 12 propeptide residues are from reference 17. The numbers above the sequences are the positions of lysine or arginine residues in P₄₆; arrows labeled P₄₁ and P₃₉ denote the sites of cleavage generating P₄₁ and P₃₉, respectively.