FIG. 3.

Autoprocessing of the stable P₃₀ domain of trypsin-digested P₄₆. P₃₀ was generated and incubated under autoprocessing conditions as described in Materials and Methods; an aliquot (10 μg) was precipitated with trichloroacetic acid, resuspended in SDS sample buffer, and subjected to SDS-PAGE (12% gel). Lane 1, unprocessed P₃₀; lane 2, autoprocessed P₃₀. Arrows a and b denote the migration positions of P₃₀ and the autoprocessed product, respectively.