TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or characteristics | Source or reference |
|---|---|---|
| E. coli strains | ||
| JM83 | ||
| UT481 | lacIq | |
| PS2479 | UT481 pPS2479 Ampra | This work |
| PS2524 | UT481 pPS2524 Ampr | This work |
| PS2569 | UT481 pPS2569 Ampr | This work |
| PS2577 | UT481 pPS2577 Ampr | This work |
| PS 2578 | UT481 pPS2578 Ampr | This work |
| Plasmids | ||
| pPS740 | pUC12 with B. megaterium wild-type gpr; Ampr | 32 |
| pPS1907 | pTZ19U with B. megaterium gpr from which all propeptide residues are deleted; Ampr | 32 |
| pPS1910 | pUC12 with B. megaterium gpr from which all propeptide residues are deleted; Ampr | 6 |
| pPS2339 | pUC12 with B. megaterium gpr lacking propeptide residues 2 to 4; Ampr | 17 |
| pPS2340 | pUC12 with B. megaterium gpr lacking propeptide residues 2 to 7; Ampr | 17 |
| pPS2341 | pUC12 with B. megaterium gpr lacking propeptide residues 2 to 10; Ampr | 17 |
| pPS2342 | pUC12 with B. megaterium gpr lacking propeptide residues 2 to 13; Ampr | 17 |
| pPS2479 | pPS1910 with B. megaterium gpr with KDALAN instead of KDIALEN in the P41→P39 cleavage site; Ampr | This work |
| pPS2524 | pPS1910 with B. megaterium gpr C115A; Ampr | This work |
| pPS2569 | pPS1910 with B. megaterium gpr S274A S276A; Ampr | This work |
| pPS2577 | pPS1910 with B. megaterium gpr S219A; Ampr | This work |
| pPS2578 | pPS1910 with B. megaterium gpr S229A; Ampr | This work |
a
Ampr, ampicillin (50 μg/ml) resistant.