FIG. 1.

Restriction map of the 6.5-kb region containing orf5-orf4-amfR-amfA and nucleotide sequences of part of this region. (A) The directions and extents of the ORFs deduced from the nucleotide sequence are shown just below the restriction map. The positions of a pair of the primers used for probes for Northern hybridization (Fig. 3) are indicated. The fragments on a low-copy-number plasmid and their ability to restore aerial mycelium formation in S. griseus HH1 are shown. (B) The nucleotide sequences of the region including the promoter in front of orf5, the termination codon of orf5 and the initiation codon of orf4, and the termination codon of orf4 and the initiation codon of amfR. Probable ribosome-binding sites for orf5 and amfR are underlined. The transcriptional start point is indicated as +1. Numbers with an arrow indicate the probes used for the gel mobility shift assay (Fig. 4).