FIG. 2.

Phenotypes of the amfR disruptant (A), schematic representation of the disrupted amfR (B), and Southern hybridization analysis of the chromosome of the amfR disruptant (C). (A) Patches were photographed after 5 days of growth at 28°C on YMPG medium. S. griseus IFO13350 (wild type [WT]) formed abundant spores, and the amfR mutant strain (ΔamfR) exhibited a Bld phenotype. The amfR disruptant harboring plasmid pAFL1 carrying the intact amfR gene with its upstream promoter region restored aerial mycelium and spore formation as abundantly as the wild-type strain. (B) Chromosomal amfR is disrupted by aphII. (C) The chromosomal DNAs from strain IFO 13350 (WT) and the amfR disruptant (Δ) were digested with BamHI or BamHI plus HindIII and hybridized with the amfR and aphII probes.