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Gel mobility shift assays for detection of a protein able to bind the promoter region of orf5-orf4-amfR. Crude extracts were prepared from the A-factor-deficient mutant strain S. griseus HH1 (upper panel) and strain HH1 grown in the presence of A-factor (lower panel) and fractionated by DEAE column chromatography. Each of the fractions was assayed by gel mobility shift assay with the 62-bp EcoRI-MluI fragment as the probe. OD 280, optical density at 280 nm.
