FIG. 6.

Gel mobility shifts of the regions upstream of orf5 caused by the purified AdpB protein. The 158-bp EcoRI-Sau3AI fragment and a series of fragments trimmed from the EcoRI end (see Fig. 1) were used. Lane 1, the 158-bp fragment (nucleotide positions −71 to 86 in Fig. 1B); lane 2, the 130-bp fragment (positions −43 to 86); lane 3, the 120-bp fragment (positions −34 to 86); lane 4, the 109-bp fragment (positions −22 to 86); lane 5, the 158-bp fragment in the presence of a 100-fold molar excess of the cold probe.