FIG. 7.
In vitro transcription from PORF5. (A) Physical map of the DNA fragments used as the templates for runoff transcription. The EcoRI-Eco47III and the EcoRI-AvaII fragments were 316 and 249 bp, respectively. The expected transcripts, 244 and 177 nucleotides (nt) are depicted as solid bars. (B) The transcripts obtained with the two templates (a in lanes 1 to 4 and b in lanes 5 and 6) in the absence of AdpB (lanes 1 and 5) and the presence of 0.1 μg (lane 2), 1 μg (lane 3), or 10 μg (lanes 4 and 6) of AdpB were analyzed by 8 M urea-PAGE.