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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: FASEB J. 2023 Oct;37(10):e23187. doi: 10.1096/fj.202300076R

Figure 3:

Figure 3:

CPAs cocktails 2, 6, 7 and 8 partially retain cardiac function of adult zebrafish hearts after 25 mins of storage at −10 °C when loaded via immersion (n = 6 animals per treatment). (A) Representative microscopic images of adult zebrafish hearts treated with different CPAs cocktails. (B) Heart rate (beats per minute – bpm) was best retained in hearts treated with CPAs 2, 6, 7 and 8 with no statistical difference seen between fresh and the time control hearts. (C) Cardiac metabolism was best retained by CPAs 6 having no statistical difference with Fresh controls. All other CPAs cocktails resulted in statistically lower cell metabolism when compared to Fresh controls and similar metabolism of hearts frozen without CPAs. All statistical data is expressed as mean ± standard deviation and analyzed via ordinary one-way ANOVA with Tukey’s multiple comparison test. * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p <0.0001.