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. 2023 Dec 28;12:e83103. doi: 10.7554/eLife.83103

Figure 6. DFP normalized TFR1 expression in MDS erythroblasts.

(A) Flow cytometry gating using TER119 and CD44 was used to delineate bone marrow erythroblasts. In these gated erythroblasts, we evaluate membrane TFR1 to demonstrate its decrease in response to DFP in MDS mice (n=7–13 mice/group) analyzed after 1 month of treatment. Tfrc mRNA expression is increased in sorted bone marrow ProE (B) from MDS mice and partially normalized in DFP-treated MDS mice. Tfrc mRNA expression is normal in sorted bone marrow BasoE (C) and PolyE (D), decreased in OrthoE (E) from MDS mice, and increased in DFP-treated relative to untreated MDS mice (n=15–18 mice/group). *p<0.05 vs. WT; **p<0.01 vs. WT; &p<0.05 vs. MDS; &&p<0.01 vs. MDS; Abbreviations: WT = wild type; MDS = myelodysplastic syndrome; DFP = deferiprone; TFR1=transferrin receptor 1; ProE = pro-erythroblasts; BasoE = basophilic erythroblasts; PolyE = polychromatophilic erythroblasts; OrthoE = orthochromatophilic erythroblasts.

Figure 6—source data 1. Source data for flow analysis of cell surface TFR1 and Tfrc in sorted bone marrow erythroblasts from wild type (WT), myelodysplastic syndrome (MDS), and DFP-treated MDS mice.

Figure 6.

Figure 6—figure supplement 1. Effect of DFP on erythroblast surface TFR1 expression in WT mice.

Figure 6—figure supplement 1.

Membrane TFR1 on bone marrow erythroblasts from WT and DFP-treated WT mice (n=5–11 mice/group). *p<0.05 vs. WT DFP; WT = wild type; DFP = deferiprone; TFR1 = transferrin receptor 1; MFI = mean fluorescence index.
Figure 6—figure supplement 1—source data 1. Source data for flow analysis of cell surface transferrin receptor 1 (TFR1) on bone marrow erythroblasts from wild type (WT) and DFP-treated WT mice.
Figure 6—figure supplement 2. Effect of DFP on erythroblast Tfrc expression in erythroblasts from WT mice.

Figure 6—figure supplement 2.

Sorted bone marrow erythroblast Tfrc mRNA expression between WT and DFP-treated WT mice (n=5–7 mice/group). *p<0.05 vs. WT DFP; WT = wild type; DFP = deferiprone; Tfrc = transferrin receptor 1.
Figure 6—figure supplement 2—source data 1. Source data for Tfrc in sorted bone marrow erythroblasts from wild type (WT) and DFP-treated WT mice.