Figure 5.

Quantitative flow virometry staining of CD38 on the surface of HIV. (A) Four different HIV isolates (IIIB, BG505, SF162, BaL) produced in PBMC were stained with PE-conjugated antibodies against CD38, CD44 and integrin β7. A stained cell culture medium control (RPMI) is shown to assess the contribution of antibodies alone (in absence of virus) to background fluorescence. Gates demarcate where the majority of viruses are anticipated to fall based on light scattering. Dotted lines indicate the level of background PE fluorescence set based on the isotype control. (B) Quantitative representation of mean fluorescent data (PE MESF) ± SD generated through the gates in two experimental replicates from flow virometry data as in (A). Solid black lines indicate levels of background capture as measured with an isotype control. Data shown are representative of five replicates for each virus stock.