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Complementation to uracil prototrophy in strain G184ASura5-11 conferred by URA5Hc DNA fragments, and diagram of plasmid pMAD10 Δura5Hc::hph disruption construct insert. Restriction endonuclease sites: A, AsnI; Ba, BamHI; Bg, BglII; H, HindIII; R, EcoRV; Sa, SalI; Sm, SmaI; St, StuI; V, AvrII; X, XhoI. Only mapped sites are shown for each fragment, and not all fragments were mapped with each enzyme.
