TABLE 1.
Transformation of H. capsulatum G184AS with ClaI-cut pMAD10, containing the Δura5Hc::hph mutant locus
| Expt | No. of hygromycin B-resistant transformants | Selectionb | Screening | No. of uracil auxotrophs |
|---|---|---|---|---|
| 1 | 379 | Hygromycin B | Uracil requirement tested by growth on medium with and without exogenous uracil | 1 |
| 1,516 (calculateda) | Hygromycin B, FOA | Uracil requirement confirmed by growth on medium with and without exogenous uracil | 2c | |
| 2 | 980 (calculateda) | Hygromycin B, FOA | Uracil requirement confirmed by growth on medium with and without exogenous uracil | 1 |
a
Based on the number of colonies arising on a 3 M–uracil–hygromycin B agarose plate (without FOA).
b
Exogenous uracil was always provided.
c
Another isolate from the original transformation plates was uracil prototrophic, and this hygromycin B-resistant transformant was presumably a spontaneous or transformation-induced FOA-resistant mutant.