Table 2.
In vitro EC50 values of 5-fluoropyrimidines and assorted anti-toxoplasma agents against T. gondii intracellular tachyzoites and HFF cells.
| Compound | tachyzoites | HFF ctotoxicity | ||
|---|---|---|---|---|
| EC50 (µM) | EC50 (µM) Cytostatic |
EC50 (µM) Cytostatic |
S.I. | |
| 5-FU | 1.13 ± 0.16 | 2.49 ± 0.43 | 3070 ± 340 | 2720 |
| 5-FUrd | 0.45 ± 0.02 | 0.11 ± 0.10 | 20.4 ± 1 | 45 |
| 5-F,2’dUrd | 0.67 ± 0.07 | 0.19 ± 0.05 | 1480 ± 510 | 2205 |
| Sulfadiazine | 12.4 ± 1.08 | ND | ND | – |
| Pyrimethamine | 16.2 | ND | ND | |
| Ara-A | 11.4 ± 1.8 | ND | ND | |
| NBMPR | 3.19 ± 0.14 | ND | ND | |
| PAO | ND | ND | 0.06 ± 0.003 | – |
SI, selectivity index (HFF cytocidal EC50/tachyzoite EC50). Each value is the average of at least three independent determinations and the SEM, except for the pyrimethamine EC50 (n=2). PAO, phenylarsine oxide.
ND, not determined.