Figure 6.

Co-localization of expressed protein and macrophages in dLN-specific cells in NHPs

(A) Fluorescent microscopic image of NHP popliteal LN 24 h post-i.m. injection of mRNA-LNP. LN was stained with anti-CD169 (red) and anti-GFP (green) antibodies. The majority of EGFP expression is detected in the SCS and medullary sinus. (B) Graph depicting flow cytometry analysis of EGFP-expressing immune cells in the dLNs and non-dLNs postinjection. Non-dLN tissue (mesenteric) and dLN tissue (axillary [left and right], popliteal [left and right], and iliac LNs [left and right]) were collected at 24 h (N = 6) postdosing. Box plot whiskers are set to contain the min and max data points. LN tissue was processed to cells, which were then stained with fluorescently labeled antibodies to delineate macrophages, cDCs, pDCs, classical monocytes, B cells, and T cells. Graph shows the level of EGFP positivity in the dLN compared to the non-dLN across all of the cell types analyzed. Area shown in insets is indicated in the low-magnification images. Scale bars, 1 mm and 100 μm.