Expression of YAP by ACL-derived cells in tendon allograft
(A) YAP protein expression in the tendon allograft was confirmed by IHC staining on day 14. Magnification ×200, scale bar 100: μm.
(B) The mRNA expression of YAP was analyzed by RT-qPCR on day 14 (n = 3).
(C) Immunofluorescence staining was performed on day 14 for YAP (red). White arrow indicates that the YAP proteins translocated into the nucleus. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ns: not significant. Statistical analysis of the data was performed using a two-tailed paired t-test. Data are shown as the mean ± SEM.