TABLE 1.
E. coli strains used in this study
| Strain | Genotype and/or descriptiona | Source or referenceb |
|---|---|---|
| Top10 | F′ λ−endA1 recA1 hsdR17 (rK− mK+) supE44 thi1 gyrA96 relA1 φ80ΔlacZΔM15Δ(lacZYA-argF) U169 deoR | Invitrogen |
| XL1-blue | endA1 hsdR17 (rK− mK+) supE44 thi1 recA1 gyrA96 relA1 lac [F′ proAB lacIqlacZΔM15 Tn10] (Tetr) | Stratagene |
| JCB495 | MC1000 recD | C. Manoil |
| CC160 | MC1000 dam | C. Manoil |
| MC4100 | araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR | Laboratory stock |
| SG1126 | SG1101 clpA::kan | 11 |
| JGT4 | MC4100 clpA::kan | P1(SG1126) × MC4100→Neor Strr Lac− |
| MC1000ΔclpB | MC1000 ΔclpB::kan | 31 |
| JGT3 | MC4100 ΔclpB::kan | P1(MC1000ΔclpB) × MC4100→Neor Strr Lac− |
| JCB23 | MC1655 F−lacZ::Tn10 zba315::kan ΔhtpG1::lacZ | 4 |
| JGT11 | MC4100 zba315::kan ΔhtpG1::lacZ | P1(JCB23) × MC4100→Neor Strr Lac+ |
| JGT1 | JCB495 Δibp1::kan | LinTr(pBRΔibp::kan 14-3) × JCB495→Neor Amps |
| JGT17 | MC4100 Δibp1::kan | P1(JGT1) × MC4100→Neor Strr Lac− |
| CG800 | C600 leu dnaK756 thr::Tn10 | S. van der Vies |
| JGT20 | MC4100 dnaK756 thr::Tn10 | P1(CG800) × MC4100→Tetr Strr Ts44 Lac− |
| CG712 | B178 zjd::Tn10 groES30 | 34 |
| JGT6 | MC4100 zjd::Tn10 groES30 | P1(CG712) × MC4100→Tetr Strr Ts44 Lac− |
| JGT44 | MC4100 zjd::Tn10 | P1(CG712) × MC4100→Tetr Strr Lac− |
| SG1162a | SG1101 clpP::cat | 11 |
| JGT19 | MC4100 clpP::cat | P1(SG1162a) × MC4100→Chlr Strr Lac− |
| JGT31 | MC4100 dnaK756 thr::Tn10 clpA::kan | P1(SG1126) × JGT20→Neor Lac− |
| JGT32 | MC4100 dnaK756 thr::Tn10 ΔclpB::kan | P1(MC1000ΔclpB) × JGT20→Neor Lac− |
| JGT30 | MC4100 dnaK756 thr::Tn10 zba315::kan ΔhtpG1::lacZ | P1(JCB23) × JGT20→Neor Lac+ |
| JGT34 | MC4100 dnaK756 thr::Tn10 Δibp1::kan | P1(JGT1) × JGT20→Neor Lac− |
| JGT27 | MC4100 zjd::Tn10 groES30 clpA::kan | P1(SG1126) × JGT6→Neor Lac− |
| JGT38 | MC4100 zjd::Tn10 groES30 ΔclpB::kan | P1(CG712) × JGT3→Neor Ts44 Lac− |
| JGT39 | MC4100 zjd::Tn10 groES30 zba315::kan ΔhtpG1::lacZ | P1(CG712) × JGT11→Neor Ts44 Lac+ |
| JGT28 | MC4100 zjd::Tn10 groES30 Δibp1::kan | P1(JGT1) × JGT6→Neor Lac− |
| BT66 | MC4100 malG(Am) malTc-Tn10 secB::Tn5 (F′ lacpro lacIq) | B. Traxler |
| JGT43 | MC4100 clpP::cat (F′ lacpro lacIq) | F′(BT66) × JGT19→Chlr Lac+ Neos Tets |
| JGT47 | MC4100 zjd::Tn10 (F′ lacpro lacIq) | F′(JGT43) × JGT44→Tetr Lac+ Chls |
| JGT61 | MC4100 dnaK756 thr::Tn10 (F′ lacpro lacIq) | F′(JGT43 × JGT20→Tetr Lac+ Chls |
| JGT49 | MC4100 zjd::Tn10 groES30 (F′ lacpro lacIq) | F′(JGT43 × JGT6→Tetr Lac+ Chls |
a
Amp, ampicillin; Neo, neomycin or kanamycin; Chl, chloramphenicol; Tet, tetracycline; Str, streptomycin. Superscripts r and s indicate resistance and sensitivity, respectively. Lac refers to the formation of blue colonies on M9 plates containing X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside) and IPTG.
b
P1 transductions are represented as P1(donor) × recipient→phenotypes used for selection and screening. Linear transformations (LinTr) are represented as LinTr(plasmid) × recipient→phenotypes used for selection and screening. F′ mating is represented as F′(donor) × recipient→phenotypes used for selection and screening. Ts44 indicates that the selected cells were unable to form colonies at 44°C.