TABLE 2.
E. coli plasmids used in this study
| Plasmid | Descriptiona | Source or reference |
|---|---|---|
| pMON18003 | pUC19 derivative carrying a 5.2-kbp fragment of the E. coli chromosome containing the ibp operon (Ampr) | 2 |
| pBRibp | pBR322 derivative encoding the ibp operon and surrounding genomic DNA (Ampr) | This study |
| pBSL14 | Encodes the gene for neomycin phosophotransferase flanked by multiple restriction sites (Neor) | 1 |
| pBRΔibp::kan14-3 | pBRibp derivative with most of the ibp operon removed by BstXI digestion and a Neor cartridge from pBSL14 inserted at this location (Ampr Neor) | This study |
| pWPC3 | pUC19 derivative encoding the clpA gene (Ampr) | 11 |
| pclpB | pBR322 derivative encoding the clpB gene (Ampr) | 31 |
| pBJ5 | pBR322 derivative encoding the htpG gene (Ampr) | 4 |
| pT7blue | pUC19-derived cloning vector (Ampr) | Novagen |
| pTb-ibp | pT7blue derivative encoding a PCR-amplified ibp operon (Ampr) | This study |
| pTG10 | pACYC184-derived cloning vector (Chlr) | 9 |
| pDnaK/J | pTG10 derivative encoding the dnaKJ operon under control of the native promoter (Chlr) | A. A. Gatenby |
| pGroESL | pTG10 derivative encoding the groE operon under control of the lac and native promoters (Chlr) | 9 |
| pς32 | pTG10 derivative encoding the rpoH gene under control of the lac promoter only (Chlr) | 33 |
| pClpA | pTG10 derivative encoding the clpA gene under control of the native promoter (Chlr) | This study |
| pClpB | pTG10 derivative encoding the clpB gene under control of the native promoter (Chlr) | This study |
| pHtpG | pTG10 derivative encoding the htpG gene under control of the native promoter (Chlr) | This study |
| pIbp | pTG10 derivative encoding the ibp operon under control of the lac and native promoters (Chlr) | This study |
a
Amp, ampicillin; Neo, neomycin or kanamycin; Chl, chloramphenicol. Superscript r indicates resistance.