Fig. 8.

Mapping of interface hotspots in the paratope. On the x-axis is shown residue IDs according to IMGT numbering and y-axis is given in percentage. The red line shows the raw residue ID occurrences i.e., the fraction of the PDBs where the specific residue IDs are found in the antibody sequence no matter if they are contact points or not. Only residue IDs that are found in minimum 10% of the PDBs were included in the analysis so rare CDR insertions are not shown. As expected, drops in background occurrence is mainly seen in the CDRs because these insertions are naturally only found in some of the antibodies. The blue bars indicate how often (in percent) a given residue ID is considered a uPR when that position is present. The value is calculated as the number of PDBs where the ID is considered a uPR relative to the number of PDBs where the given ID is available in the sequence. The data is shown for protein-binding Fv antibodies (a), peptide-binding Fv antibodies (b) and protein-binding sdAbs (c).