Table 1.
Lectin- and Immuno-labelling reagents and conditions for F. hepatica NEJs
| Lectin/Primary antibody | Specificity | Secondary labelling | Counter-staining |
|---|---|---|---|
| Griffonia simplicifolia – GSL I (1:200) | Terminal α-Gal | FITC-Streptavidin (1:200) | Phalloidin-(TRITC)(200 μg/ml) |
| Terminal α-GalNAc (Tn-antigen). | |||
| Arachis hypogaea –PNA (1:500) | Galβ1,3GalNAc | ||
| (T-antigen) | |||
| Erythrina cristagalli – ECL (1:200) | Galβ1,4GlcNAc (LacNAc) | ||
| βGal | |||
| Lactose | |||
| Triticum vulgaris – WGA (1:200) | Terminal βGlcNAc | ||
| Neu5Ac | |||
| Concanavalin A – FITC-ConA (1:200) | α-Man | N/A | |
| Glc | |||
| Monoclonal anti-Man3/Man5 (1:500) | Man3 and Man5 | FITC-goat anti-human IgG (1:200) | |
| Anti- PC (TEPC)(1:500) | PC | FITC-goat anti-mouse IgA (1:200) | |
| Polyclonal rabbit anti-FhCL3 (1:500) | FhCL3 | FITC-Goat anti-rabbit IgG (1:200) | |
| Rabbit pre-immune sera (1:500) | N/A |
Gal, Galactose; GalNAc, N-acetylgalctosamine; GlcNAc, N-acetylglucosamine; Man, Mannosee; FhCL3, F. hepatica cathepsin L3.