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. 2023 Aug 18;15(12):1070–1080. doi: 10.1111/1753-0407.13457

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© 2023 The Authors. Journal of Diabetes published by Ruijin Hospital, Shanghai Jiaotong University School of Medicine and John Wiley & Sons Australia, Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Isolation and characterization of small extracellular vesicles (sEVs) from serum of diabetic patients with or without cardiac dysfunction. (A) sEVs were isolated from serum of control, DM, and DM‐CAD patients by commercial kits with 6 independent replicates. Electron microscopy images of sEVs isolated from serum of control, DM, and DM‐CAD. (B) CD9 and TSG101 of sEVs from different groups were detected by western blots. Scale bar: 100 nm. (C) Particle distributions of sEVs by nanosight particle tracking system. (D) Lipid classes and relative abundance detected in lipidomic analysis. DM, diabetic patients without cardiovascular diseases; DM‐CAD, diabetic patients with cardiovascular diseases.