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SqgA is a sulfoquinovosidase. (a) HPLC mass spectra (triple quadrupole, QqQ) chromatograms showing Flavobacterium sp. FlSqgA catalyzed conversion of SQGro (total ion chromatogram MS2 of m/z = 317.08) to SQ (total ion chromatogram MS2 of m/z = 243.00) at time (t) = 0, 30, 60, and 120 min (b–d) Michaelis–Menten and Lineweaver–Burk plots for reaction rates measured for FlSqgA catalyzed hydrolysis of SQGro, α-PNPSQ, and β-PNPSQ, respectively.
