TABLE 3.
Localization and description of ORFs and noncoding elements in pMT1
| ORF or noncoding element | Position no. | Size (amino acid residues) | Strand or direction | Description (homologue by BLAST) |
|---|---|---|---|---|
| ORFs | ||||
| 1 | 87–1109 | 340 | Direct | Transposase |
| 2 | 1109–1888 | 259 | Direct | Transposase |
| 3 | 2022–2618 | 198 | Complement | Similar to hypothetical E. coli protein |
| 4 | 2932–5514 | 860 | Complement | Similar to tail fiber protein gp37 |
| 5 | 5901–6518 | 205 | Complement | Unknown |
| 6 | 6571–11049 | 1,492 | Complement | Putative protein, similar to lambda host-specific protein J and Northern European squid neurofilament-like protein |
| 7 | 11188–11739 | 183 | Complement | Unknown |
| 8 | 11763–12521 | 252 | Complement | Similar to hypothetical Coxiella burnetii protein (motif: RGD cell attachment site) |
| 9 | 12553–13251 | 232 | Complement | Similar to phage lambda minor tail protein L |
| 10 | 13341–13676 | 111 | Complement | Unknown |
| 11 | 13718–18295 | 1,525 | Complement | Putative protein, similar to hypothetical Haemophilus influenzae protein HI1514 |
| 12 | 18653–18970 | 105 | Complement | Unknown |
| 13 | 19030–19776 | 248 | Complement | Unknown |
| 14 | 19851–20207 | 118 | Complement | Unknown (Block hit: complement C1Q domain signature) |
| 15 | 20236–20616 | 126 | Complement | Unknown (Block hit: NSF attachment protein) |
| 16 | 20700–21044 | 114 | Complement | Unknown (Block hit: geminivirus AR1 coat protein) |
| 17 | 21142–21975 | 277 | Complement | Unknown (Block hit: histone H5 signature) |
| 18 | 21975–22274 | 99 | Complement | Unknown |
| 19 | 22453–22884 | 143 | Complement | Similar to glucan endo-1,3-β-d-glucosidase |
| 20 | 23190–24065 | 291 | Complement | Unknown |
| 21 | 24092–24409 | 105 | Complement | Unknown |
| 22 | 24537–24977 | 146 | Complement | Unknown |
| 23 | 25015–26253 | 412 | Complement | Unknown |
| 24 | 26623–27831 | 402 | Complement | Unknown (motif: ATP/GTP binding motif A [P loop]) |
| 25 | 27882–28523 | 213 | Complement | Unknown |
| 26 | 28719–28985 | 88 | Complement | Unknown (motif: RGD cell attachment site) |
| 27 | 28995–29885 | 296 | Complement | Unknown |
| 28 | 30138–30776 | 212 | Complement | Similar to ABC transporter (motif: ATP/GTP binding motif A [P loop]) |
| 29 | 30773–31441 | 222 | Complement | Unknown (motif: aminoacyl-RNA synthetase class II, signature 2) |
| 30 | 31441–32121 | 226 | Complement | Unknown |
| 31 | 32285–33763 | 492 | Direct | Unknown |
| 32 | 33766–34044 | 92 | Direct | Unknown |
| 33 | 34269–34526 | 85 | Direct | Unknown |
| 34 | 34531–35058 | 175 | Direct | Similar to S. typhimurium repressor of phase 1 flagellin gene |
| 35 | 35382–36032 | 216 | Direct | Unknown |
| 36 | 36982–37464 | 160 | Complement | Unknown |
| 37 | 37669–37950 | 93 | Complement | Unknown |
| 38 | 38797–39384 | 195 | Complement | Unknown |
| 39 | 40132–42015 | 627 | Complement | Unknown (motifs: ABC transporter, aminoacyl-tRNA synthetase class II signature 2, ATP/GTP binding motif A [P loop]) |
| 40 | 42278–43231 | 317 | Complement | Unknown |
| 41 | 45161–45385 | 74 | Complement | Unknown |
| 42 | 46550–47614 | 354 | Direct | Similar to E. coli replication protein A (repA) |
| 43 | 48184–48384 | 66 | Complement | Unknown |
| 44 | 48396–48656 | 86 | Complement | Unknown |
| 45 | 49291–49587 | 98 | Complement | Unknown |
| 46 | 50211–50996 | 261 | Complement | Unknown |
| 47 | 51336–52412 | 358 | Complement | Similar to recA (motif: ATP/GTP binding motif A [P loop]) |
| 48 | 52681–53550 | 289 | Complement | Similar to Streptococcus pneumoniae DNA Pol Ia |
| 49 | 53686–54714 | 342 | Complement | Unknown (motif: RGD cell attachment site) (Block hit: T. cruzi P2 protein signature) |
| 50 | 54834–55265 | 143 | Complement | Unknown |
| 51 | 55810–56373 | 187 | Direct | Unknown |
| 52 | 56403–56846 | 147 | Complement | Unknown |
| 53 | 56843–60250 | 1135 | Complement | Similar to DNA Pol III alpha subunit |
| 54 | 60548–61783 | 411 | Complement | Similar to CobS protein |
| 55 | 61881–64235 | 784 | Complement | Similar to CobT protein |
| 56 | 67074–67588 | 175 | Complement | F1 capsule antigen |
| 57 | 67669–70170 | 833 | Complement | F1 capsule anchoring protein |
| 58 | 70195–70971 | 258 | Complement | caf1M |
| 59 | 71299–72204 | 301 | Direct | caf1R |
| 60 | 72719–73024 | 101 | Complement | Unknown |
| 61 | 73545–74582 | 347 | Complement | Similar to DNA ligase |
| 62 | 74658–75437 | 259 | Complement | Transposase |
| 63 | 75437–76459 | 340 | Complement | Transposase |
| 64 | 76861–77118 | 85 | Complement | Unknown |
| 65 | 77419–78048 | 209 | Complement | Unknown |
| 66 | 79222–80430 | 402 | Direct | Transposase |
| 67 | 80899–81921 | 340 | Direct | Transposase |
| 68 | 82120–82437 | 105 | Complement | Similar to E. coli yhgA |
| 69 | 82617–82988 | 123 | Complement | Similar to hypothetical protein |
| 70 | 83297–83752 | 151 | Direct | Similar to E. coli hypothetical protein and reverse transcriptase-like protein |
| 71 | 83997–85595 | 532 | Complement | Murine toxin |
| 72 | 87149–87340 | 63 | Direct | Unknown |
| 73 | 87363–88220 | 285 | Complement | Putative protein, similar to E. coli yhgA |
| 74 | 90045–91250 | 401 | Direct | Similar to phage P7 parA |
| 75 | 91247–92218 | 323 | Direct | Similar to phage P7 parB |
| 76 | 94893–95534 | 213 | Direct | Similar to adenine DNA methyltransferase |
| 77 | 95766–96185 | 139 | Direct | Unknown (Block hit: NSF attachment site) |
| 78 | 96239–97018 | 259 | Direct | Unknown |
| 79 | 97417–97923 | 168 | Direct | Similar to antirestriction protein |
| 80 | 98635–98865 | 76 | Direct | Similar to E. coli hypothetical protein |
| 81 | 98938–100947 | 669 | Direct | Similar to Rhizobium meliloti protein and S. sonnei protein |
| Noncoding elements | ||||
| 1–1954 | Forward | IS100 | ||
| 38040–38757 | Reverse | IS200 | ||
| 46565–47425 | Forward | IncF plasmid RepFIB replicon | ||
| 74592–76545 | Reverse | IS100 |
Pol, polymerase.