Skip to main content
. 2023 Nov 13;26(12):108374. doi: 10.1016/j.isci.2023.108374
REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and virus strains

Bacterial isolates (n = 51) P. damicornis mucus Available at Table S3

Biological samples

Pocillopora damicornis Ocyan Reef coral farm (Itú, São Paulo, Brazil) N/A

Chemicals, peptides, and recombinant proteins

Marine Agar 2216 BD DIFCO™ Cat#212185
Marine Broth 2216 BD DIFCO™ Cat#279110
Agar BD DIFCO™ Cat#281210

Critical commercial assays

DNeasy PowerSoil Pro Kit QIAGEN Cat#47016
Genomic DNA Purification Kit Wizard® Cat#A1120

Deposited data

Raw amplicon sequencing data This study/NCBI’s SRA PRJNA934364
16S rRNA sequences from all 51 isolates This study/NCBI’s GenBank Available at Table S3

Oligonucleotides

515F Apprill et al.74 5′-GTGYCAGCMGCCGCGGTAA-3′
806R Apprill et al.74 5′-GGACTACNVGGGTWTCTAAT-3′
27F Weisburg et al.75 5′-AGAGTTTGATCCTGGCTCAG -3′
1492R Weisburg et al.75 5′-CTACGGCTACCTTGTTACGA -3′

Software and algorithms

QIIME2 (v.2022.2) Bolyen et al.76 https://forum.qiime2.org/
DADA2 Callahan et al.77 https://benjjneb.github.io/dada2/
Phyloseq McMurdie and Holmes78 https://github.com/joey711/phyloseq
DESeq2 Love et al.79 doi:10.18129/B9.bioc.DESeq2
Vegan (v.2.6–4) https://rdrr.io/cran/vegan/ https://github.com/vegandevs/vegan
ggplot2 (v.3.4.0) https://cran.r-project.org/web/packages/ggplot2/index.html https://github.com/tidyverse/ggplot2
BioEdit 7.2 Hall80 https://bioedit.software.informer.com/7.2/

Other

Centrifugal Microfilters Whatman™ Centrex™ Cat#32-10467015