Bacterial and virus strains |
|
Bacterial isolates (n = 51) |
P. damicornis mucus |
Available at Table S3
|
|
Biological samples |
|
Pocillopora damicornis |
Ocyan Reef coral farm (Itú, São Paulo, Brazil) |
N/A |
|
Chemicals, peptides, and recombinant proteins |
|
Marine Agar 2216 |
BD DIFCO™ |
Cat#212185 |
Marine Broth 2216 |
BD DIFCO™ |
Cat#279110 |
Agar |
BD DIFCO™ |
Cat#281210 |
|
Critical commercial assays |
|
DNeasy PowerSoil Pro Kit |
QIAGEN |
Cat#47016 |
Genomic DNA Purification Kit |
Wizard® |
Cat#A1120 |
|
Deposited data |
|
Raw amplicon sequencing data |
This study/NCBI’s SRA |
PRJNA934364 |
16S rRNA sequences from all 51 isolates |
This study/NCBI’s GenBank |
Available at Table S3
|
|
Oligonucleotides |
|
515F |
Apprill et al.74
|
5′-GTGYCAGCMGCCGCGGTAA-3′ |
806R |
Apprill et al.74
|
5′-GGACTACNVGGGTWTCTAAT-3′ |
27F |
Weisburg et al.75
|
5′-AGAGTTTGATCCTGGCTCAG -3′ |
1492R |
Weisburg et al.75
|
5′-CTACGGCTACCTTGTTACGA -3′ |
|
Software and algorithms |
|
QIIME2 (v.2022.2) |
Bolyen et al.76
|
https://forum.qiime2.org/ |
DADA2 |
Callahan et al.77
|
https://benjjneb.github.io/dada2/ |
Phyloseq |
McMurdie and Holmes78
|
https://github.com/joey711/phyloseq |
DESeq2 |
Love et al.79
|
doi:10.18129/B9.bioc.DESeq2
|
Vegan (v.2.6–4) |
https://rdrr.io/cran/vegan/ |
https://github.com/vegandevs/vegan |
ggplot2 (v.3.4.0) |
https://cran.r-project.org/web/packages/ggplot2/index.html |
https://github.com/tidyverse/ggplot2 |
BioEdit 7.2 |
Hall80
|
https://bioedit.software.informer.com/7.2/ |
|
Other |
|
Centrifugal Microfilters |
Whatman™ Centrex™ |
Cat#32-10467015 |