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. 2023 Nov 30;18(6):100872. doi: 10.1016/j.ajps.2023.100872

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© 2023 Shenyang Pharmaceutical University. Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 2 — The endocytosis and cytotoxicity of INP@TPt. (A) Confocal scanning microscopy observation of INP@Rh B uptake by A2780DDP cells. Scale bar: 25 µm. (B) Time-dependent measurement of the intracellular level of INP@RhB in A2780DDP cells by flow cytometry. (C) The Pt count in A2780DDP cells after incubation with CDDP, INP@Pt(IV), NP@TPt, or INP@TPt at 37 °C. Viability curves of A2780 cells (D) and A2780DDP cells (E) after treatment with different concentrations of CDDP, Pt(IV), TPt, INP@Pt(IV), NP@TPt, INP@TPt for 48 h. (F) Apoptosis analysis of PBS, CDDP, Pt(IV), TPt, INP@Pt(IV), NP@TPt, or INP@TPt (3 µM Pt) on A2780DDP cells for 48 h. **P<0.05, ***P<0.001.