Skip to main content
. 2023 Oct 27;9(12):2205–2215. doi: 10.1021/acscentsci.3c01102

Figure 3.

Figure 3

(A) 4b maintained the viability of lung (A549) cells upon treatment with 15 wt % PA14 csn. Live/dead imaging with A549 cells challenged with 15% (v/v) of PA14 csn with and without 4b. Green signals: living cells; red signals: dead cells. Red signals in some cases were lost because the detached cells were washed away after the rinsing step with PBS. Scale bar: 200 μm for images. (B) Probability of survival of the Galleria mellonella larvae infected with wt PA14: The survival of larvae infected with wt PA14 is shown after treatment with 1 nmol 4b, 10.7 nmol tobramycin, or a combination of both. The survival in the PBS-4b or tobramycin or combination of both treated groups was 100%. The statistical difference between groups treated with 1 nmol 4b + 10.7 nmol tobramycin and treated with only wt PA14 is p = 0.0005 (log-rank). Each curve represents results of three independent experiments. wt PA14: wild-type PA14; ΔPA14: LasB knockout PA14; and csn: culture supernatant.