Table 1. Plasmids Used in This Study.
| plasmid | application | template | origin |
|---|---|---|---|
| pICH41308 | level 0 cloning backbone for pc4cl and phchs | Addgene#4799827 | |
| pICH47742 | level 1 cloning backbone for pc4cl | Addgene#4800127 | |
| pICH47761 | level 1 cloning backbone for phchs | Addgene#4800327 | |
| pFTK013 | PpcbC Pc21g21380 promoter for pc4cl | P. rubens DS5446829 | Addgene#17128528 |
| pFTK081 | TactA ANIA_06542 P. rubens terminator for pc4cl | pDSM-JAK-10830 | Addgene#17135328 |
| pFTK012 | P40s AN0465 promoter for phchs | pDSM-JAK-10830 | Addgene#17128428 |
| pFTK076 | Ttif35 Pc22g19890 terminator for phchs | pDSM-JAK-10830 | Addgene#17134828 |
| pCP1_45 | level 1 vector with PgpdA-ergA-Tamds as the terbinafine selection marker | P. rubensDS5446831 | Pohl et al.23 |
| pFL_0_1_Pc4CL | level 0 vector with pc4cl CDS | pETDuet:: pc4cl | this study |
| pFL_0_2_PhCHS | level 0 vector with phchs CDS | pETDuet:: phchs32 | this study |
| pFL_1_1_Pc4CL | level 1 vector with TU for donor DNA | this study | |
| pFL_1_2_PhCHS | level 1 vector with TU for donor DNA | this study | |
| pET28a/Cas9-Cy | Cas9 protein overexpression | Addgene#5326133 |
CDS: coding sequence; TU: transcription unit.