(A) Metabolic pathway for oxidation products from acetyl
RCF oil
oligomers in P. putida strain CJ781. Base treatment
of the oxidation products hydrolyzed acetyl groups from aromatic monomers,
yielding a mixture of syringaldehyde, syringate, vanillin, and vanillate.
Syringaldehyde and syringate are converted to biomass (growth) via
3-O-methylgallate, gallate, and 4-oxalomesaconate.
Vanillin and vanillate are converted to muconate via protocatechuate
and catechol. (B) Strain CJ781 cultivated in M9 minimal medium with
1 mM/each of the model compounds syringaldehyde, syringate, vanillin,
vanillate. (C) Strain CJ781 cultivated in M9 minimal medium with 10%
v/v oxidation products from acetyl RCF oil. Gallate and catechol were
not detected at significant concentrations in any of the experiments,
and 4-oxalomesaconate was not measured. All cultures contained 5 mM
glucose at time zero, and glucose was fed to a concentration of 5
mM every 24 h to support growth. Error bars represent the standard
deviation from the mean of three biological replicates. Numerical
data are provided in Table S15, and additional
reaction replicates for the experiment in (C) are shown in Figure S12.