REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
anti-mouse CD3 | eBioscience | 500A2 |
anti-mouse CD4 | eBioscience | RM4–5 |
anti-mouse CD8 | BD bioscience | 53–6.7 |
anti-mouse CD45 | BD bioscience | 30-F11 |
anti-mouse CD4 | BD Bioscience | GK1.5 |
anti-mouse CD44 | eBioscience | IM7 |
anti-mouse CD62L | eBioscience | MEL-14 |
anti-mouse CD69 | eBioscience | H1.2F3 |
anti-mouse GR1 | eBioscience | RB6–8C5 |
anti-mouse Rorgt | BD Bioscience | Q31–378 |
anti-mouse Ki-67 | BD Bioscience | B56 |
anti-mouse IFN gamma | eBioscience | XMG1.2 |
anti-mouse TNF alpha | eBioscience | MP6-XT22 |
anti-mouse FoxP3 | eBioscience | FJK-16s |
anti-mouse Tbet | eBioscience | 4B10 |
ant-mouse GATA3 | eBioscience | TWAJ |
anti-mouse TCRb | eBioscience | H57–597 |
anti-mouse PD1 | eBioscience | J43 |
anti-mouse CD86 | BD Bioscience | GL1 |
anti-mouse CD11b | Biolegend | M1–70 |
anti-mouse CD11c | Biolegend | N418 |
anti-mouse CD103 | Biolegend | 2E7 |
anti-mouse CD64 | Biolegend | X54–5/7.1 |
anti-mouse CD80 | Biolegend | 16–10A1 |
anti-mouse IA/IE | eBioscience | M5/114.15.2 |
anti-mouse SiglecF | BD Bioscience | E50–2440 |
anti-human CD3 | Biocare Medical | LN10 |
anti-human CD8 | CST | C8/144 |
anti-human PD1 | CST | D4W2J |
anti-human FOXP3 | Thermo Scientific | 236A/E7 |
anti-mouse CD11c | Biolegend | N418 |
anti-mouse CD4 | Biolegend | GK1.5 |
anti-mouse CD103 | Biolegend | M290 |
anti-mouse NQO1 | Sigma Aldrich | HPA007308 |
Chemicals, Peptides, and Recombinant Proteins | ||
IFN-alpha | R&D | 12100–1 |
Deposited Data | ||
scRNA-seq and RNA-seq data | GEO | GSE241482 |
Experimental Models: Cell Lines | ||
KrasLSL-G12D/+; p53fl/fl lung adenocarcinoma cells (1233, 1234) | Tyler Jacks Lab | N/A |
Experimental Models: Organisms/Strains | ||
B6.129S(C)-Batf3tm1Kmm/J | Jackson Laboratories | 013755 |
Keap1 fl/fl | Taconic | 8799 |
KrasLSL-G12D/+; p53fl/fl | Jackson Laboratories | 032435 |
Oligonucleotides | ||
UTY | UTY-F: 5’-GAGGTTTTGTGGCATGGGAG -3’; UTY-R: 5’-TGCAGAAGATAACGAAGGAGCTA-3’ |
|
Blvrb | Blvrb-F: 5’-CCGATGTGGACAAGACTGTG-3’; Blvrb-R: 5’-TCGGACATTACTGTAGTGGGACT-3’ |
|
Nqo1 | Nqo1-F: 5’-AGCGTTCGGTATTACGATCC-3’ Nqo1-R: 5’-AGTACAATCAGGGCTCTTCTCG-3’; |
|
Slc7a11 | Slc7a11-F: 5’-GATTCATGTCCACAAGCACAC-3’; Slc7a11-R: 5’-GAGCATCACCATCGTCAGAG-3’; |
|
ISG15 | ISG15-F 5’- GGAACGAAAGGGGCCACAGCA-3’; ISG15-R 5’- CCTCCATGGGCCTTCCCTCGA-3’ |
|
IFN beta | IFNβ -F 5’ – GCTCCTGGAGCAGCTGAATG-3’; IFNβ – R 5’ – CGTCATCTCCATAGGGATCTTGA-3’; |
|
CXCL10 | CXCL10 – F 5’ – TGATTTCAAGCTTCCCTATGGC – 3’; CXCL10 – R 5’ – ATTTTCTGCCTCATCCTGCT – 3’; |
|
IRF7 | IRF7 – F 5’ – GAAGAGGCTGGAAGACCAACTTC-3’; IRF7 – R 5’- GAATTGTCTTGGCGCAAGATAA; |
|
Software and Algorithms | ||
GraphPad Prism v9 | N/A | |
FlowJo | N/A |