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. Author manuscript; available in PMC: 2024 Sep 15.
Published in final edited form as: Org Lett. 2023 Sep 5;25(36):6767–6772. doi: 10.1021/acs.orglett.3c02736

Figure 5.

Figure 5.

Photoaffinity labeling of proteins using diazirine-functionalized peptides. Panel A) Labeling of rFTase: Lane 1: No diazirine (19 instead of 20a), + UV; Lane 2: Probe (20a), – UV; Lane 3: Probe (20a), + UV; Lane 4: Probe (20a) + UV, + competitor (16). Panel B) Labeling of SmgGDS isoforms: Lane 1: SmgGDS558, No diazirine (19 instead of 20a), + UV; Lane 2: SmgGDS558, Probe (20a), – UV; Lane 3: SmgGDS558, Probe (20a), + UV; Lane 4: SmgGDS607 No diazirine (19 instead of 20a), + UV; Lane 5: SmgGDS607, Probe (20a), – UV; Lane 6: SmgGDS607, Probe (20a), + UV. Note all reactions with SmgGDS proteins also contained yFTase that was used to enzymatically prepare 20a from 19 and 1 prior to photolysis. In Panel A, Lane 1, and Panel B, Lanes 1 and 4, diazirine 1 was omitted to prevent the formation of 20a from 19. Compound 1 was present in all other samples.