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Maximum Likelihood tree non-collapsed of concatenated genes 16S rRNA, COI, RAG1 and TYR taken from GenBank and novel sequences. Numbers on nodes are bootstrap values (see Materials and Methods section for details). Green shadow corresponds to the ingroup. Pristimantissimilaris sp. nov. in red, Pristimantis sp. 3 from Bolivia in purple and Pristimantis sp. from Cusco in blue.
