Skip to main content
. 2023 Dec 21;120(52):e2318274120. doi: 10.1073/pnas.2318274120

Fig. 4.

Fig. 4.

Most PER2 bodies diffuse freely in the nucleus and are formed by a mechanism distinct from overexpression-caused LLPS condensates. (A) Representative image of nuclear PER bodies in PER2-EGFP KI cell under light-sheet fluorescence microscopy at hour 10 after synchronization. (Scale bar: 5 μm.) (B) Representative movement tracks of PER bodies. 1 to 4: freely diffusing tracks. 5: immobile tracks. 6: a diffusing PER body became immobile. (C) The averaged MSD of PER bodies computed from data collected from three cells. Data are presented as means ± SEM. (D) Mean speeds of different tracks (n = 3 cells). (E) Duration of immobility of PER2 bodies of the identified immobile PER2 bodies (data collected from three cells). (F) Representative image of nuclear PER bodies at 10 min after treatment with DMSO or 1,6-hexanediol in KI cells at hour 10 after synchronization. (G) Comparison of nuclear PER2-EGFP fluorescence intensities of KI cells at 10 min after treatment with DMSO or 1,6-hexanediol at hour 10 after synchronization. Data are presented as median fluorescence intensity in each cell. n = 14 to 18 cells. (H) Western blot of PER2-EGFP in the indicated cells at hour 10 after synchronization. The arrow indicates the phosphorylated PER2-EGFP species. * a background band. (I) Representative Airyscan images of PER bodies in the indicated cells at hour 10 after synchronization. (J) Nuclear PER2 body numbers in the indicated cells at 10 h and 16 h after dexamethasone synchronization.