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. 2023 Dec 21;120(52):e2318274120. doi: 10.1073/pnas.2318274120

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Copyright © 2023 the Author(s). Published by PNAS.

This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 5. — Airyscan imaging analyses of dual fluorescence reporter U2OS cells in which the endogenous PER2 and BMAL/CRY1 are tagged by EGFP and mScarlet-I, respectively. (A) Diagrams showing the design of the knock-in donor plasmids. (B) Time course Airyscan fluorescence imaging of PER2 and BMAL1 in fixed indicated KI cells after dexamethasone synchronization. (Scale bar: 5 μm.) (C) Time course Airyscan imaging of PER2 and CRY1 in fixed double KI cells after dexamethasone synchronization. (D and E) Airyscan fluorescence imaging of PER2 and BMAL1/CRY1 in live double KI cells.