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. 2023 Dec 18;120(52):e2313009120. doi: 10.1073/pnas.2313009120

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Copyright © 2023 the Author(s). Published by PNAS.

This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 2. — The FVII SP drives effective protein secretion in vitro as validated by mRNA delivery. (A) FVII-mCherry mRNA was synthesized by IVT and encapsulated in LNPs for delivery. (B) Time-dependent (at 100 ng mRNA per well) and dose-dependent (at 72 h) mCherry secretion in Huh7 cells. (C) Dose-dependent (at 72 h) mCherry secretion in different cell lines after treatment with mRNA LNPs in a 96-well plate. At the noted time points, mCherry signal was quantified by plate reader. (D) Obvious mCherry signal was observed in the medium at day 3 after FVII-mCherry mRNA formulation treatment. HEK293T cells in a 96-well plate were treated by mRNA LNPs with different doses and imaged by confocal microscopy (exposure time, 1/6 s for 24 h and 1/15 s for 72 h).