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. 2024 Jan;30(1):172–176. doi: 10.3201/eid3001.231164

Figure 2.

Figure 2

Phylogenetic analysis of MPXV sequences from a cluster of mpox cases described in Kwango Province, Democratic Republic of the Congo. A) MPXV global phylogeny showing that the Kwango Province outbreak cluster belongs to clade I MPXV. B) Phylogenetic analysis of MPXV genome sequences from the reported cases and clade I MPXV sequences from Central Africa. Posterior support values are shown at branch points. DNA was extracted at Institut National de Recherche Biomédicale using a QIAGEN DNA Mini Kit (https://www.qiagen.com) from blood samples and subsequently screened for MPXV with an orthopoxvirus-specific real-time PCR assay. Whole-genome sequencing was attempted on samples from the index case by next-generation sequencing. The library preparation was performed using Illumina DNA Prep with Enrichment (https://www.illumina.com), and the libraries were enriched for MPXV using biotinylated custom probes synthesized by Twist Biosciences (https://www.twistbioscience.com). Note that 23MPX098 (or 23MPX0245V) and 23MPX099 (or 23MPX0245C) are vesicle and crust samples from case-patient 1. Scale bar indicates number of substitutions per site. DRC, Democratic Republic of the Congo; hMPXV, human MPXV; MPXV, monkeypox virus.