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. 2024 Jan;30(1):163–167. doi: 10.3201/eid3001.231003

Table. List of oligonucleotide primers used to examine emergence of novel norovirus GII.4 strains on 3 continents* .

Primer names Sequence, 5′ → 3′ Orientation Nested reverse transcription PCR
NV6e ACC AYT WTG ATG CAG ACT A Forward Round 1
NV6f ACC AYT ATG ATG CTG ATT A Forward Round 1
NV6g ATC AYT ATG ATG CWG AYT A Forward Round 1
NV357a CGC CAG TCC AGG AGT CCA AAA TY Reverse Round 1
NV378 GCT TAC GAA TGT GAG CGA A Reverse Round 2

*Primers were used to amplify complete ORF2 and ORF3 genes of GII.4 San Francisco strains from Gabon. ORF, open reading frame