Table. List of oligonucleotide primers used to examine emergence of novel norovirus GII.4 strains on 3 continents* .
Primer names | Sequence, 5′ → 3′ | Orientation | Nested reverse transcription PCR |
---|---|---|---|
NV6e | ACC AYT WTG ATG CAG ACT A | Forward | Round 1 |
NV6f | ACC AYT ATG ATG CTG ATT A | Forward | Round 1 |
NV6g | ATC AYT ATG ATG CWG AYT A | Forward | Round 1 |
NV357a | CGC CAG TCC AGG AGT CCA AAA TY | Reverse | Round 1 |
NV378 | GCT TAC GAA TGT GAG CGA A | Reverse | Round 2 |
*Primers were used to amplify complete ORF2 and ORF3 genes of GII.4 San Francisco strains from Gabon. ORF, open reading frame