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. 2024 Jan;30(1):168–171. doi: 10.3201/eid3001.230756

Table 1. NAI antibody titers detected in postinfection ferret antiserum against influenza A(H1N1), A(H1N1)pdm09, and A(H5N1) viruses, Hong Kong, China.

Postinfection
ferret antiserum†
NAI antibody titers*
Homologous virus California/09 A (H1N1)pdm09 Spoonbill/HK/22 A(H5N1)
A/USSR/90/1977 320 <10 <10
A/Chile/01/1983 320 <10 <10
A/Singapore/06/1986 320 <10 <10
A/Texas/36/1991 320 <10 <10
A/Brisbane/59/2007 1,280 <10 <10
A/California/04/2009 2,560 2,560 320–640

*NAI antibody response was determined using recombinant A(H6N1) viruses carrying the N1 proteins derived from A(H1N1), A(H1N1)pdm09, or A(H5N1) viruses that were generated as described previously (10). Although we used recombinant H6N1 viruses in ELLA assays to avoid interference of hemagglutinin-reactive antibodies, we might not completely prevent the interference of cross-reactive antibodies that bind to the stalk of H6 protein and inhibit neuraminidase activity through stearic hindrance. NAI, neuraminidase inhibition. †One ferret antiserum against each of the influenza A(H1N1) viruses and 6 ferret antiserum against the influenza A(H1N1)pdm09 were used.