Figure 4. Expression of lymphatic EC and pro-lymphangiogenic cytokines in wild type (WT) and RAMP1^−/− mice after feeding normal diet (ND) or high-fat diet (HFD). (A) mRNA expression levels of Vegfr3, Prox1, Lyve-1, Vegfc, and Vegfd in the jejunum from WT and RAMP1^−/− mice fed ND or HFD. Data are expressed as the mean±SD. *p<0.05, **p<0.01, and ***p<0.001; NS: Not significant. (B) Immunofluorescence staining for VEGF-C (green) and F4/80 (red) in the lacteals from WT mice after feeding ND or HFD. Cell nuclei were stained with DAPI (blue). Arrowheads indicate merged cells. Scale bar: 10 μm.