Fig 1. Genome structure of the recombinant LC16m8 expressing NiV surface glycoproteins.

Schematic diagrams of the procedure for constructing recombinant LC16m8 (upper) and genome structures of the foreign genes inserted into the LC16m8 genome (lower). Plasmid pRecB5R.1 expresses the mCherry and XGPRT genes under the vaccinia early and late promoter (P) and contains the homologous sequences that flank the B5R gene (UP and DOWN). The genes of interest (GOIs), which were cloned into the pRecB5R.1, were transferred to the LC16m8 genome through homologous recombination. The selection marker genes XGPRT and mCherry were self-excised from the intermediate recombinant viruses by intragenomic homologous recombination between the UP and shorter sequence homologous to UP (U’). The dotted lines indicate the expected locations of the homologous recombination sites. The final recombinant viruses express the NiV G or F gene, while the complementary genome DNA expresses the EGFP gene.