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. 2005 Mar;79(6):3509–3516. doi: 10.1128/JVI.79.6.3509-3516.2005

FIG. 5.

FIG. 5.

Localization of OPN protein in the intestine. Nine-day-old BALB/c mouse pups were orally inoculated with either RRV or TNC. Samples were harvested at 24 h p.i., and immunohistochemistry using polyclonal rabbit anti-rat OPN was performed. OPN was found localized to small intestinal epithelial cells with supranuclear and luminal accentuation in RRV- but not TNC-inoculated pups. Magnification, ×20. (A) Sham-inoculated mouse small intestine. (B) RRV-infected opn+/+ mouse intestine. (C) RRV-infected opn+/+ mouse stained for OPN. (D) opn−/− mouse infected with RRV and stained for OPN. Note the extensive vacuolization (compared to panel A) but the absence of OPN staining in the RRV-infected null mutants.