FIG. 1.

(A) Construction of CSFV infectious cDNA clones pBIC and pCSIC, using amplicon fragments 5′, M, and 3′. Corresponding restriction sites and nucleotide numbers are depicted (pBIC/pCSIC). (B) Growth curve of CSFV Brescia and CS and viruses derived from ICs BIC and CSIC. Primary macrophage cell cultures were infected (MOI = 0.1) and harvested at the indicated time points. Samples were titrated on SK6 cells with virus detected by immunohistochemistry as described in Material and Methods. Data are means and standard errors from three independent experiments.