FIG. 1.

There is a significant increase in the percentage of nonfunctional cells in the lung over time following infection with SV5. BALB/c mice were infected intranasally with 10⁶ PFU of WT rSV5, and lymphocytes were isolated from the lungs of mice on d7, d12, or d40 postinfection. Lymphocytes were stained with M_285-293/L^d MHC-I tetramer or stimulated with 1 μM M_285-293 peptide and stained for intracellular IFN-γ (see Materials and Methods). Lymphocytes were pooled from three to four mice before staining. As a negative control, cells were stimulated in the absence of peptide. The numbers represent the percentages of CD8⁺ T cells that are either tetramer positive or IFN-γ⁺. The data shown are representative of at least four independent experiments.