TABLE 1.
Function of lung lymphocytes from mice infected with SV5a
| Day n | Expt | Lung lymphocyte result
|
||
|---|---|---|---|---|
| % Tetramer positive | % IFN-γ+ | % Functional | ||
| d7b | ||||
| 1 | 6.7 | 6.9 | >100 | |
| 2 | 7.6 | 7.0 | 92 | |
| 3 | 7.9 | 7.9 | 100 | |
| d12c | ||||
| 1 | 44.3 | 19.3 | 44 | |
| 2 | 42.6 | 21.6 | 51 | |
| 3 | 40.5 | 21.1 | 52 | |
a
Adult BALB/c mice were infected with SV5, and lung lymphocytes were analyzed directly ex vivo at d7 and d12 postinfection. Antigen-specific T cells were identified by the ability to bind Ld-M285-293 tetramer. In addition, cells were stimulated in vitro for 5 h with M285-293 peptide and their ability to produce IFN-γ was assessed by ICS. Numbers represent the percentages of CD8+ T cells that were positive. The percent functional values were obtained by dividing the values for IFN-γ by tetramer values.
b
Data are from four pooled mice per experiment.
c
Data are from three pooled mice per experiment.