FIG. 3.

Analysis of BFLF2 RNA expression. (A) Northern blot analysis of RNA extracted from B95-8 cells at different times after treatment with TPA and sodium butyrate (T/B), with and without treatment with phosphonoacetic acid (T/B+PAA). Twenty micrograms of total RNA was separated on a 1.2% agarose-6% formaldehyde gel, and the blot was subsequently probed with the oligonucleotide RG3 as described in Materials and Methods. Equal RNA loading was assessed by β-actin hybridization. (B) 5′ and 3′ RACE analysis of BFLF2 transcripts. The transcription start and stop sites of the BFLF2 ORF as they were mapped by 5′-3′ RACE assay are shown. Genomic coordinates are given with respect the B95-8 viral strain.