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. 2005 Mar;79(6):3713–3727. doi: 10.1128/JVI.79.6.3713-3727.2005

FIG. 4.

FIG. 4.

(A) BFLF2 and BFRF1 coprecipitate in B95-8 cells. Cellular extracts from B95-8 cells and B95-8 cells activated with TPA and sodium butyrate (T/B) for 48 h were immunoprecipitated with rabbit anti-BFRF1 polyclonal antibody and with MAb C1 against BFLF2. Immunoprecipitated proteins were subjected to electrophoresis and transferred to nitrocellulose filters. The resulting immunoblots were probed with anti-BFRF1 MAb E7 and anti-BFLF2 MAb C1. Lanes 1 and 4: extracts from uninduced and induced B95-8 cells immunoprecipitated with protein G-conjugated beads without antibody. Lanes 2 and 5: extracts immunoprecipitated with anti-BFRF1 polyclonal antibody. Lanes 3 and 6: extracts immunoprecipitated with anti-BFLF2 MAb C1. (B) BFLF2 and BFRF1 coprecipitate in 293 transfected cells. Cellular extracts from 293 cells transiently transfected with CMV-BFRF1 (F1) or CMV-BFLF2 (F2) or cotransfected with CMV-BFRF1 and CMV-BFLF2 (F1+F2) were immunoprecipitated with anti-BFRF1 polyclonal antibody (IP anti F1) and with the anti-BFLF2 MAb C1 (IP anti F2). After electrophoresis, the resulting immunoblots were probed with anti-BFRF1 MAb E7 and with anti-BFLF2 MAb C1.