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. 2005 Mar;79(6):3713–3727. doi: 10.1128/JVI.79.6.3713-3727.2005

FIG. 9.

FIG. 9.

(A) Lamin B (LB) coprecipitates with BFRF1 and BFLF2 in B95-8 cells. Cellular extracts from untreated B95-8 cells (−) and B95-8 cells induced with TPA plus butyrate (+) were immunoprecipitated (IP) with anti-lamin B polyclonal antibody. Immunoprecipitated proteins were run on polyacrylamide gels, and the resulting immunoblots were probed with the anti-lamin B antibody and with MAbs directed against BFRF1 and BFLF2. (B) Lamin B coprecipitates with BFRF1 and BFLF2 in 293 cells. 293 cells were transfected with CMV-BFRF1 (293+F1) and CMV-BFLF2 (293+F2) and cotransfected with CMV-BFRF1 and CMV-BFLF2 (293+F1+F2). At 24 h following transfection, cells were lysed and the resulting extracts were immunoprecipitated with anti-lamin B antibody. After electrophoresis, proteins were transferred to nitrocellulose membranes and analyzed by Western blotting as described for panel A. (C) Indirect immunofluorescence of BFRF1, BFLF2, and lamin B expression in induced B95-8 cells and in 293 cells transfected with CMV-BFRF1 (293+F1) or with CMV-BFLF2 (293+F2). (D) Confocal microscopy showing that BFRF1 colocalizes with lamin B, whereas BFLF2, in the absence of BFRF1, does not colocalize with lamin B. Bars, 10 μm.